BioFlo 4500 is an industrial fermentor/bioreactor with the power, versatility and advanced controls for microbial, insect and mammalian cell culture, in applications ranging from research through small-scale production. A wide array of options enable configuration to your individual process requirements, without the high cost and delays normally associated with purchasing a custom fermentor.  The systems most powerful feature is its advanced control capability, driven by NBS’ ML-6100™ multi-loop controller with touch-screen interface . With the ML-6100, on-screen entry of process control loops, sterilization cycles and calibration routines is remarkably intuitive, making the BioFlo 4500 exceptionally easy to operate. Almost any auxiliary process device, from sensors to biochemical analyzers, can be integrated with the fermentor without need for additional hardware, software or a service technician. In fact, every facet of this compact system has been designed with efficiency in mind. An open-frame design provides easy access to all piping, valves, regulators and filters; and assignable, peristaltic pumps with Easy-Load® pump heads speed tube loading. The system is fully cGMP compliant. For process flexibility and operating simplicity the BioFlo 4500 has no equal.  
BioFlo 4500 has been thoroughly inspected and tested  .  

VESSEL

Total Capacity

30 Liters

Working Volume

5 - 15 Liters

7 - 22.5 Liters

Aspect Ratio (H:D)

1.98 : 1

2.03 : 1

Geometry

Cylindrical with hemispherical bottom dish

Pressure Rating

Vessel is ASME-coded, rated for 40 psig (2.8 kg/cm2) Jacket rated for 50 psig (3.5 kg/cm2)

Viewing Window

Rectangular window, sidewall mounted above and below the maximum working level. Area of visibility is 8.5” High x 1.25” Wide  (21.6 x 3.2 cm)

Baffles

(4) 316L stainless steel removable baffles

Dimensions

9.48” I.D. x 18.8” H (24.08 x 47.78 cm)

10.79” I.D. x 22” H (27.41 x 55.88 cm)

MATERIALS & FINISH

Vessel

316L stainless steel, polished to an internal finish of 15 - 20 Ra and an external finish of 25 - 30 Ra. Electropolishing optional

Process Piping

316L stainless steel, orbitally welded. All valves and associated components are of 316L stainless steel

Gaskets/O-Rings

Gaskets of Silicone; O-Rings of EPDM

IMPELLERS

Outer Diameter

3.75” (9.53 cm)

4.31“ (10.95 cm)

Type

(2) Six-bladed 316L stainless steel, Rushton blade, standard. Marine blade, pitched blade, spin filter and basket impeller, optional

NOZZLES

Headplate

(7) 28 mm ports, 19 mm I.D. plugged; (1) exhaust port, tri-clamp; (1) combination filling/light port, tri-clamp; (1) bearing housing; (1) rupture disk 316L stainless steel, 40 psi (2.7 bar) piped to bottom of console, tri-clamp

Upper Side Wall

(4) 19 mm Ingold-type ports, plugged; (1) overlay port, plugged; (1) sparge port, with multi-orifice ring sparger

Lower Side Wall

(2) 19 mm Ingold-type ports, plugged; (1) Thermowell, 19 mm; (3) 25 mm Ingoldtype ports, plugged

Jacket

(1) Jacket water in; (1) Jacket water out; (1) Jacket pressure relief valve

Bottom

(1) 19 mm harvest/drain valve poppet-type with .25” (0.63 cm) quick connect

AGITATION

Drive

1 HP, AC motor, top-entry

Sensor

Fiber optical photo interrupter

Range

50 - 1,000 rpm, +1 rpm

Control

PID regulation of speed via microprocessor feedback circuit

Bearing Housing

Single mechanical seal, standard. Double mechanical seal optional

TEMPERATURE

Sensor

Platinum RTD in thermowell

Control

(4) Cartridge heaters at the bottom of the vessel. Cooling water is admitted to the jacket through the temperature control valve and the flow control valve. PID regulation, with PWM

Range

5°C above coolant supply temperature to 85°C, ±0.2°C

Shift

1°C/minute in growth phase in a range from 30°C to 45°C

AIR FLOW

Inlet Filter

Sterilizable-In-Place (SIP) absolute 0.2 µ filter with 316L stainless steel housing

Outlet Filter

SIP depth filter with 316L stainless steel housing. Absolute 0.2 µ filter optional

Control

Thermal Mass Flow Controller, PID regulation